Key Product Details
Product Summary for Human CD3 epsilon Antibody
Scientific Data Images for Human CD3 epsilon Antibody
Detection of Human CD3 epsilon by Western Blot.Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry.Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Recombinant Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).
CD3 epsilon in Human PBMCs.CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Recombinant Monoclonal Antibody (Catalog # MAB100R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; (NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Applications for Human CD3 epsilon Antibody
Sample: Human peripheral blood lymphocytes
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
T Cell Stimulation
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
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Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
- Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Product Specific Notices for Human CD3 epsilon Antibody
For research use only