CD38 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD38, Monoclonal Antibody (Catalog # MAB24041) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

CD38 in Human Hodgkin Lymphoma via seqIF™ staining on COMET™

CD38 was detected in immersion fixed paraffin-embedded sections of human Hodgkin Lymphma using Mouse Anti-Human CD38, Monoclonal Antibody (Catalog # MAB24041) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

CD38 in Human Lymph Node via seqIF™ staining on COMET™

CD38 was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human CD38, Monoclonal Antibody (Catalog # MAB24041) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

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Applications for Human CD38 Antibody

Application

Recommended Usage

COMET

1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil, lymph node and Hodgkin Lymphoma

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human CD38 (Catalog # 2404‑AC), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, RPMI 8226 human multiple myeloma cell line, and CEM human T-lymphoblastoid cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD38

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)⁺ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP⁺, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5).

References

Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
Sun, L. et al. (1999) J. Cell Biol. 146:1161.
Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
Kato, I. et al. (1995) J. Biol. Chem. 270:30045.

Long Name

Cluster of Differentiation 38

Alternate Names

ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase

Entrez Gene IDs

952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)

Gene Symbol

CD38

UniProt

P28907

Additional CD38 Products

Product Documents for Human CD38 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD38 Antibody

For research use only

Citations
Reviews

Human CD38 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB24041

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD38 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD38 Antibody

CD38 in Human Tonsil via seqIF™ staining on COMET™​

CD38 in Human Hodgkin Lymphoma via seqIF™ staining on COMET™​

CD38 in Human Lymph Node via seqIF™ staining on COMET™​

Detection of CD38 in human tonsil.

Detection of Human CD38 by Western Blot.