Human CD36/SR-B3 Antibody

Name: Human CD36/SR-B3 Antibody
Brand: R&D Systems
SKU: MAB19552

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB19552

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36/SR‑B3 Gly30-Asn439 Accession # P16671
Applications:	Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 877346
Specificity:	Detects human CD36/SR‑B3 in direct ELISAs.

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Applications for Human CD36/SR-B3 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10-50 µg/mL	See below
Immunohistochemistry	5 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD36/SR-B3 Antibody

	Western Blot Detection of Human CD36/SR‑B3 by Western Blot. Western blot shows lysates of human placenta tissue and human platelets. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD36/SR-B3 Mono-clonal Antibody (Catalog # MAB19552) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD36/SR-B3 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunohistochemistry CD36/SR‑B3 in Human Heart. CD36/SR-B3 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human CD36/SR-B3 Monoclonal Antibody (Catalog # MAB19552) at 5 µg/mL for 1 hour at room temperature followed by incubation with Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Simple Western Detection of Human CD36/SR‑B3 by Simple Western^TM. Simple Western lane view shows lysates of human platelets, loaded at 0.5 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 138 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19552). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Simple Western Detection of Human CD36/SR‑B3 by Simple Western^TM. Simple Western lane view shows lysates of human adipose tissue, loaded at 0.2 mg/mL. A specific band was detected for CD36/SR‑B3 at approximately 125 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human CD36/SR‑B3 Monoclonal Antibody (Catalog # MAB19552). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta ‑amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

Item	Value
References	Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. Husemann, J. et al. (2002) Glia 40:195. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Long Name	Scavenger Receptor Class B, Member 3
Entrez Gene IDs	948 (Human); 12491 (Mouse)
Alternate Names	BDPLT10, CD36, CD36 antigen, CD36 antigen (collagen type I receptor, thrombospondin receptor), CD36 molecule (thrombospondin receptor), CD_antigen: CD36, CHDS7, Collagen R, FAT, Fatty acid translocase, GP3B, GP4, GPIIIb, GPIV, Glycoprotein IIIb, PAS IV, PAS-4, PAS-4 protein, PASIV, Platelet collagen receptor, SCARB3, SR-B3, SRB3, Thrombospondin R, Thrombospondin receptor, cluster determinant 36, platelet glycoprotein 4, scavenger receptor class B, member 3

Citations for Human CD36/SR-B3 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD36/SR-B3 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Hu C et al. (0), Nanoparticle biointerfacing by platelet membrane cloaking. Natur526(7571):118-21.
PMID: 26374997

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