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Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
Catalog # MAB9929 | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu113
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Product Summary for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
Immunogen
Human CD3
Specificity
Detects Human CD3 based on Teplizumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Teplizumab.
This product is for research
use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
Detection of CD3 in Human PBMCs by Flow Cytometry.
Human PBMCs were stained with (A) Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929) or (B) no primary antibody, followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Detection of CD3 in Human Jurkat cell line by Flow Cytometry.
Human Jurkat cell line was stained with Human CD3 (Teplizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9929, filled histogram) or Normal Human IgG control antibody (Catalog # 1-001-A, open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMCs and Jurkat cell line
Sample: Human PBMCs and Jurkat cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified from cell culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: CD3
Alternate Names
CD3, CD3E, CD_antigen: CD3e, CD3 antigen, delta subunit, CD3-DELTA, CD3-epsilon, CD3-GAMMA, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3G, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE
Gene Symbol
CD3E
Product Documents for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
Product Specific Notices for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
For research use only
FAQs for Human CD3 (Research Grade Teplizumab Biosimilar) Antibody
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Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody.
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Q: What is a Biosimilar antibody?
A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody. It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody. For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.
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Q: What is the light chain of Human CD3 (Research Grade Teplizumab Biosimilar) Antibody, Catalog # MAB9929?
A: The light chain is kappa.
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Q: What is the optimal working concentration of the antibody?
A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.
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