Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma, rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha, rhIL-6 R, rhIL-7 R, rhIL-9 R, and rhIL-10 R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CD25/IL-2R alpha Antibody

CD25/IL‑2 R alpha in Human PBMCs.

CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody.

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223). The ND₅₀ is typically 0.5-1.0 µg/mL.

Applications for Human CD25/IL-2R alpha Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin

Western Blot

1 µg/mL
Sample: Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IL-2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 0.5-1.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL-2.

Reviewed Applications

Read 1 review rated 3 using MAB223 in the following applications:

Block/Neutralize (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Gene Symbol

IL2RA

UniProt

P01589

Additional CD25/IL-2R alpha Products

Product Documents for Human CD25/IL-2R alpha Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CD25/IL-2R alpha Antibody

For research use only

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Human CD25/IL-2R alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB223

Key Product Details

Species Reactivity

Validated:

Cited:

Applications