Human CD20 mFluor™ Violet 450-Conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4225MFV450
![CD20 Antibody (396444) [mFluor Violet 450 SE]](https://resources.bio-techne.com/images/products/fab4225mfv450_mouse-cd20-mab-396444-mfluor-violet-450-se-flow-cytometry-532024105444..jpg "Detection of CD20 in PBMC lymphocytes by Flow Cytometry")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 396444
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human CD20
Met1-Pro297
Accession # P11836
Met1-Pro297
Accession # P11836
Specificity
Detects human CD20. Stains human CD20 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD20 mFluor™ Violet 450-Conjugated Antibody
Detection of CD20 in PBMC lymphocytes by Flow Cytometry
PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD20 mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB4225MFV450) or (B) unstained . View our protocol for Staining Membrane-associated Proteins.Applications for Human CD20 mFluor™ Violet 450-Conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.1-1 µg/106 cells
Sample: PBMC lymphocytes
Sample: PBMC lymphocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD20
CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
UniProt
Additional CD20 Products
Product Documents for Human CD20 mFluor™ Violet 450-Conjugated Antibody
Product Specific Notices for Human CD20 mFluor™ Violet 450-Conjugated Antibody
mFluorTM is a trademark of AAT Bioquest, Inc.
For research use only
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