Skip to main content

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 45531

Product Summary for Human CCR5 Antibody

Immunogen

NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681

Specificity

Detects human CCR5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCR5 Antibody

Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCR5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB182) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
CCR5 antibody in Human Dendritic Cells by Immunocytochemistry (ICC).

CCR5 in Human Dendritic Cells.

CCR5 was detected in immersion fixed human dendritic cells using Human CCR5 Monoclonal Antibody (Catalog # MAB182) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutraliza-tion by Human CCR5 Antibody.

Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutraliza-tion by Human CCR5 Antibody.

Recombinant Human CCL3/MIP‑1a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP‑1a (40 ng/mL) is neutralized (green line) by increasing concentrations of Human CCR5 Monoclonal Antibody (Catalog # MAB182). The ND50 is typically 5-20 µg/mL.

Applications for Human CCR5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human dendritic cells

Neutralization

Measured by its ability to neutralize CCL3/MIP‑1 alpha-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 40 ng/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.3 using MAB182 in the following applications:

Published Applications

Read 28 publications using MAB182 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for macrophage tropic HIV-1 infection.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

UniProt

Product Documents for Human CCR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCR5 Antibody

For research use only

Loading...
Loading...

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents