Human CCL23/Ck beta8-1 Alexa Fluor® 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF508V
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CCL23/Ckβ8‑1
Met46-Asn137
Accession # AAD10846
Met46-Asn137
Accession # AAD10846
Specificity
Detects CCL23/Ck beta8‑1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human CCL23/Ck beta8-1 Alexa Fluor® 405-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Neutralization
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CCL23/Ck beta 8-1
CCL23 (SCY A23), a splice variant of CK beta8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta8-1, CK beta8/MPIF-1 lacks a 17 aa residue stretch (Leu 47‑Gly 63) present in CCL23 and has a unique arginine at residue 46. CK beta8-1/CK beta8 (MPIF-1) and leukotactin/MIP-1 delta form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1 delta), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta8-1 and CK beta8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta8 (MPIF-1), the truncated form of CK beta8-1 (aa 46‑132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta8-1 (aa 22‑132).
Additional CCL23/Ck beta 8-1 Products
Product Specific Notices for Human CCL23/Ck beta8-1 Alexa Fluor® 405-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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