Detects human Cathepzin X/Z/P in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin A, rhCathepsin B, rhCathepsin C, rhCathepsin D, and rhCathepsin L is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cathepsin X/Z/P Biotinylated Antibody

Cathepsin X/Z/P in Human Breast Cancer Tissue.

Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Cathepsin X/Z/P Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF934) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Cathepsin X/Z/P Biotinylated Antibody

Application

Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cathepsin X/Z/P (Catalog # 934-CY)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin X/Z/P

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

References

Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
Menard, R. et al. (2001) Biol. Chem. 382:839.
Therrien, C. et al. (2001) Biochemistry 40:2702.
Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
Guncar, G. et al. (2000) Structure Fold Des. 8:305.
Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.

Alternate Names

Cathepsin P, Cathepsin Z, CTSZ

Entrez Gene IDs

1522 (Human); 64138 (Mouse)

Gene Symbol

CTSZ

UniProt

Q9UBR2

Additional Cathepsin X/Z/P Products

Product Documents for Human Cathepsin X/Z/P Biotinylated Antibody

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SDS

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Product Specific Notices for Human Cathepsin X/Z/P Biotinylated Antibody

For research use only

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Human Cathepsin X/Z/P Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF934

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity