Detects human Cathepsin V in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Cathepsin H and recombinant human (rh) Cathepsin K is observed and no cross-reactivity with rhCathepsin B, C, L, O, S, Z, rmCathepsin B, C, F, or Z is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Cathepsin V Antibody

Cathepsin V was detected in Breast Tumor

Cathepsin V was detected in immersion fixed paraffin-embedded sections of breast tumor using Mouse Anti-Human Cathepsin V Monoclonal Antibody (Catalog # MAB1080) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and lysosomes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cathepsin V Antibody

Application

Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin V (Catalog # 1080-CY), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Cathepsin V (Catalog # 1080-CY)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin V

Cathepsin V (also known as Cathepsin L2 and U) is a cysteine protease of the papain family (1). It is expressed as a proenzyme in lysosomes, which can be autocatalytically converted to the mature form at pH 4.0 (2). Cathepsin V shows high amino acid identity with Cathepsin L (77% for the proenzymes and 80% for the mature enzymes) (3). However, Cathepsin V is specifically expressed in the thymus, testis, and corneal epithelium whereas Cathepsin L is expressed throughout the body.

References

Turk, V. et al. (2001) EMBO J. 20:4629.
Bromme, D. et al. (1999) Biochemistry 38:2377.
Somoza, J.R. et al. (2000) Biochemistry 39:12543.

Alternate Names

Cathepsin L2, Cathepsin U, CTSL2

Entrez Gene IDs

1515 (Human)

Gene Symbol

CTSV

UniProt

O60911

Additional Cathepsin V Products

Product Documents for Human Cathepsin V Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cathepsin V Antibody

For research use only

Citations
Reviews

Human Cathepsin V Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1080

Key Product Details

Species Reactivity

Validated:

Cited:

Applications