Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

Name: Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1049

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF1049

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Cathepsin A/Lysosomal Carboxypeptidase A Ala29-Tyr480 Accession # P10619
Applications:	Immunohistochemistry, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human Cathepsin A/Lysosomal Carboxypeptidase A in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Cathepsin A is observed and less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S, and rhCathepsin X/Z/P is observed.

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Applications for Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE)
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human prostate subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

Item	Value
Entrez Gene IDs	5476 (Human); 19025 (Mouse)
Alternate Names	CTSA, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, EC 3.4.16, EC 3.4.16.5, GLB2, GSL, Lysosomal Carboxypeptidase A, PPCA, PPGBprotective protein for beta-galactosidase (galactosialidosis), Protective protein cathepsin A, Protective protein for beta-galactosidase, beta-galactosidase 2, beta-galactosidase protective protein, cathepsin ANGBE, lysosomal protective protein

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Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Citations for Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

Product Documents for Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

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