Human Caspase-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6215
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Primate - Cercopithecus aethiops (African Green Monkey), Transgenic Mouse
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 661228
Product Specifications
Immunogen
E.coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466
Asn120-Asp297
Accession # P29466
Specificity
Detects human Caspase-1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Caspase-1 Antibody
Detection of Human, Mouse, and Rat Caspase‑1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Caspase‑1 in THP‑1 Human Cell Line.
Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Caspase-1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using MAB6215 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-1
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.
Additional Caspase-1 Products
Product Documents for Human Caspase-1 Antibody
Product Specific Notices for Human Caspase-1 Antibody
For research use only
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