Human CARD9 Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5248AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CARD9
Specificity
Detects human CARD9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) CARD6, rhCARD8, rhCARD11, rhCARD14, and rhCARD10 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human CARD9 Alexa Fluor™ Plus 594-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CARD9
CARD9 (caspase recruitment domain-containing protein 9) is a 70 kDa member of the CARD module family of proteins. It is expressed in myeloid lineage cells such as macrophages, neutrophils and dendritic cells and mediates activation of p38 and Jnk, as well as NF-kappa B by interacting with BCL10 and MALT1 in a PKC-independent manner. Human CARD9 is 536 amino acids (aa) in length. It contains one CARD domain (aa 6‑98), two coiled-coil regions (aa 117‑277 and 332‑419) and a phosphorylation site at Ser460. CARD9 dimerizes with itself and BCL10. Multiple potential splice variants are noted for CARD9. There are 35 aa and 39 aa substitutions for aa 479‑536, a seven aa substitution for aa 360‑536 and an eleven aa substitution for aa 482‑536. Over aa 9‑256, human CARD9 is 88% aa identical to mouse CARD9.
Long Name
Caspase Recruitment Domain Containing Protein 9
Alternate Names
CANDF2, caspase recruitment domain family, member 9, caspase recruitment domain-containing protein 9, hCARD9
Gene Symbol
CARD9
UniProt
Additional CARD9 Products
Product Specific Notices for Human CARD9 Alexa Fluor™ Plus 594-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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