Human Carbonic Anhydrase VI Antibody

Name: Human Carbonic Anhydrase VI Antibody
Brand: R&D Systems
SKU: AF2939

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2939

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI Gln18-Asn308 Accession # EAW71606
Applications:	Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase (CA) 5A and rhCA5B is observed and less than 1% cross-reactivity with rhCA1, rhCA2, rhCA3, rhCA4, rhCA7, rhCA8, rhCA9, rhCA10, rhCA12, rhCA13, and rhCA14 is observed.

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Applications for Human Carbonic Anhydrase VI Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	1 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Carbonic Anhydrase VI Antibody

	Western Blot Detection of Human Carbonic Anhydrase VI by Western Blot. Western blot shows human saliva, human milk, and lysates of HSC-3 human oral squamous cell carcinoma cell line and LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Simple Western Detection of Human Carbonic Anhydrase VI by Simple Western^TM. Simple Western lane view shows lysates of human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).

Item	Value
References	Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50. Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382. Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635. Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380. Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.
Entrez Gene IDs	765 (Human); 298657 (Rat)
Alternate Names	CA-VI, CA6, Carbonate dehydratase VI, Carbonic Anhydrase VI, EC 4.2.1.1, Gustin, MGC21256, Salivary carbonic anhydrase, Secreted carbonic anhydrase, carbonic anhydrase 6, carbonic anhydrase VIGUSTIN

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Human Carbonic Anhydrase VI Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Carbonic Anhydrase VI Antibody

Scientific Data Examples for Human Carbonic Anhydrase VI Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Carbonic Anhydrase VI

Citations for Human Carbonic Anhydrase VI Antibody

Product Documents for Human Carbonic Anhydrase VI Antibody

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