Detects endogenous human CaM Kinase II delta isoforms in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKII alpha, CaMKII beta, or CaMKII gamma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human CaM Kinase II delta Antibody

Detection of Human CaM Kinase II delta by Western Blot.

Western blot shows lysates of human heart, liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CaM Kinase IId Monoclonal Antibody (Catalog # MAB4176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CaM Kinase IId isoforms at approximately 50 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human CaM Kinase II delta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart, liver and kidney tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaM Kinase II delta

Calcium/calmodulin-dependent protein kinase type II delta (CaMKII delta) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and delta, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKII delta expression is ubiquitous and has been identified as the predominant CaMKII isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.

Long Name

Calcium/calmodulin-dependent Protein Kinase II delta Subunit

Alternate Names

CAMK2D

Entrez Gene IDs

817 (Human); 108058 (Mouse); 24246 (Rat)

Gene Symbol

CAMK2D

UniProt

Q13557

Additional CaM Kinase II delta Products

Product Documents for Human CaM Kinase II delta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CaM Kinase II delta Antibody

For research use only

Citations
Reviews

Human CaM Kinase II delta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4176

Key Product Details

Species Reactivity

Validated:

Cited:

Applications