Human Calsyntenin-2 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5480U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Calsyntenin-2
Ser21-Thr834 (Val366Ile)
Accession # Q9H4D0
Ser21-Thr834 (Val366Ile)
Accession # Q9H4D0
Specificity
Detects human Calsyntenin-2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Calsyntenin-1 and rhCalsyntenin-3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human Calsyntenin-2 Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Calsyntenin-2
Calsyntenin-2 (CS-2) is a 120-135 kDa member of the Alcadein family of molecules. It is expressed in the ER/Golgi, and the plasma membranes of almost all neurons. It is a calcium-binding protein that interacts with APP and X11L. In this regard, it appears to regulate APP cleavage and gene activation, and likely impacts post-synaptic signaling. Notably, when cleaved in a manner similar to APP, its intracellular fragment antagonizes AICD gene activation. Mature human CS-2 is a 935 amino acid (aa) type I transmembrane protein. It contains two cadherin domains (aa 44-280) in its extracellular region (aa 21-831) and a 103 aa cytoplasmic tail. Proteolytic cleavage by ADAM10 occurs between His803 and Leu804 to generate a 105-110 kDa extracellular fragment. Additional processing of the transmembrane fragment by gamma-secretase creates a 3 kDa fragment (aa 804-834) plus a 25 kDa cytoplasmic protein. Potential isoform variants show a premature truncation after Gln608, and the use of an alternative start site at Met409. Over aa 21-834, human CS-2 shares 95% aa identity with mouse CS-2.
Alternate Names
Alcadein-gamma, Calsyntenin2, CLSTN2, CS2, CSTN2
Gene Symbol
CLSTN2
UniProt
Additional Calsyntenin-2 Products
Product Specific Notices for Human Calsyntenin-2 Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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