Detects human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Cadherin‑8, rhE‑Cadherin, rhP‑Cadherin, or rhVE‑Cadherin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Cadherin-17 Antibody

Detection of Human Cadherin-17 by Western Blot.

Western blot shows lysates of human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cadherin‑17 in Human Ascending Colon.

Cadherin-17 was detected in immersion fixed paraffin-embedded sections of human ascending colon using Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).

Cadherin‑17 in Human Transverse Colon.

Cadherin-17 was detected in immersion fixed paraffin-embedded sections of human transverse colon using Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue).

Applications for Human Cadherin-17 Antibody

Application

Recommended Usage

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ascending and transverse colon

Western Blot

1 µg/mL
Sample: Human colon tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-17

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.

Alternate Names

Cadherin17, CDH17

Entrez Gene IDs

1015 (Human); 12557 (Mouse)

Gene Symbol

CDH17

UniProt

CAA58231

Additional Cadherin-17 Products

Product Documents for Human Cadherin-17 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cadherin-17 Antibody

For research use only

Citations
Reviews

Human Cadherin-17 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1032

Key Product Details

Species Reactivity

Validated:

Cited:

Applications