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Human Biglycan Antibody

Bio-Techne includes R&D Systems | Catalog # AF2667

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AF2667-SP
AF2667

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse, N/A

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Biglycan Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Biglycan
Asp38-Lys368
Accession # P21810

Specificity

Detects human Biglycan in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Biglycan Antibody

Biglycan antibody in Human Kidney Array by Immunohistochemistry (IHC-P).

Biglycan in Human Kidney Array.

Biglycan was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Biglycan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2667) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse Biglycan by Immunocytochemistry/Immunofluorescence

Detection of Mouse Biglycan by Immunocytochemistry/Immunofluorescence

LDL binding to the vessel wall in vitro following treatment with Site B peptide or enzymatic digestion of proteoglycan GAG chains. Tissue sections of carotid arteries from wild‐type mice with intimal hyperplasia were incubated with human LDL. Bound LDL was detected using anti‐apoB antibody. (A) LDL binding to tissue sections pre‐incubated with positively charged SiteB peptide (white circles) or neutrally charged SiteB KE peptide (gray circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). (B) LDL binding to tissue sections pre‐treated with the GAG‐degrading enzymes chondroitinase (white circles) or heparinase (white dot circles). Black circles=no pre‐treatment. White triangles=no LDL incubation (buffer only). Data was analyzed using Mann–Whitney rank sum test. Graph bars show median values. P < 0.05 is regarded significant, n = 5–8 in each group. (C) Multi‐immunostaining for biglycan (red, left panels) and perlecan (green, right panels) of tissue sections from the injured region (upper panels) and the uninjured region (lower panels) 3 weeks after carotid injury in a wild‐type mouse. Nuclei are stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28716818), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Biglycan Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney and skin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Biglycan (Catalog # 2667-CM)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 16 publications using AF2667 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Biglycan

Biglycan is a small secreted proteoglycan belonging to the leucine-rich repeat (LRR) protein family. It contains a central 12 LRR repeat domain flanked by small cysteine clusters at either side. Two glycosaminoglycans (GAG) chains are attached near the amino terminus. Biglycan binds BMP-4 and Chordin to regulate BMP-4 signaling. It may also be involved in collagen fiber assembly. Human Biglycan shares 98% amino acid sequence homology with mouse Biglycan, 97% sequence homology with rat and bovine Biglycan and 96% sequence homology with canine and equine Biglycan.

Alternate Names

BGN, DSPG1, PG-S1, SLRR1A

Entrez Gene IDs

633 (Human); 12111 (Mouse)

Gene Symbol

BGN

UniProt

Additional Biglycan Products

Product Documents for Human Biglycan Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Biglycan Antibody

For research use only

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