Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB85602AFP647

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FAB85602AFP647-100UG
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1021209

View all beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human beta‑1,3‑Glucuronyltransferase 1/B3GAT1

Specificity

Detects human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta1-3Gal beta1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

References

  1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
  2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
  3. Kakuda, S. et al. (2005) Glycobiology 2:203.
  4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
  5. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

Alternate Names

CD57, GLCATP, GLCUATP, HNK1, LEU7

Entrez Gene IDs

27087 (Human); 76898 (Mouse); 117108 (Rat)

Gene Symbol

B3GAT1

UniProt

Q9P2W7

Additional beta-1,3-Glucuronyltransferase 1/B3GAT1 Products

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Certificate of Analysis

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Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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