Key Product Details
Product Summary for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody
Accession # Q9P2W7
Scientific Data Images for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody
Detection of Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 by Western Blot.Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85602) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta-1,3-Glucuronyltransferase 1/B3GAT1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat beta‑1,3‑Glucuronyltransferase 1/B3GAT1 by Simple WesternTM.Simple Western lane view shows lysates of mouse brain tissue and rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for beta‑1,3‑Glucuronyltransferase 1/B3GAT1 at approximately 48 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85602) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
beta‑1,3‑Glucuronyltransferase 1/B3GAT1 in Human Brain.beta-1,3-Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human beta-1,3-Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB85602) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Applications for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)
Sample: Mouse brain tissue and Rat brain tissue
Sample: Human cerebellum tissue
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Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-1,3-Glucuronyltransferase 1/B3GAT1
- Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
- Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
- Kakuda, S. et al. (2005) Glycobiology 2:203.
- Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
- Wu, Z.L. et al. (2011) Glycobiology 21:727.
Product Specific Notices for Human beta‑1,3‑Glucuronyltransferase 1/B3GAT1 Antibody
For research use only