Western blot shows lysates of human heart ventricle tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human beta-1 Adrenergic R/ADRB1 Monoclonal Antibody (Catalog # MAB10119) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta-1 Adrenergic R/ADRB1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human beta-1 Adrenergic R/ADRB1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart ventricle tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1 Adrenergic R/ADRB1

The beta-1 Adrenergic Receptor (ADRB1) is a 477 aminoacids long and seven-transmembrane domain protein that belongs to the G protein-coupled receptor (GPCR) 1 family. The adrenergic receptors, including ADRB1, are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. ADRB1 binds epinephrine and norepinephrine with approximately equal affinity. ADRB1 is localized at the plasma membrane. It is also found in the Golgi upon Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) overexpression.

Long Name

Beta-1 Adrenergic Receptor

Alternate Names

ADRB1, ADRB1R, beta-1 AdrenergicR, beta1 AdrenergicR

Entrez Gene IDs

153 (Human); 11554 (Mouse); 24925 (Rat)

Gene Symbol

ADRB1

UniProt

P08588

Additional beta-1 Adrenergic R/ADRB1 Products

Product Documents for Human beta-1 Adrenergic R/ADRB1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human beta-1 Adrenergic R/ADRB1 Antibody

For research use only

Citations
Reviews

Human beta-1 Adrenergic R/ADRB1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10119

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all beta-1 Adrenergic R/ADRB1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human beta-1 Adrenergic R/ADRB1 Antibody

Detection of Human beta‑1 Adrenergic R/ADRB1 by Western Blot.