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Human BAK Antibody

Bio-Techne includes R&D Systems | Catalog # MAB8161

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MAB8161
MAB8161-SP

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 564305

Product Summary for Human BAK Antibody

Immunogen

E. coli-derived recombinant human BAK
Pro20-Asn124
Accession # Q16611

Specificity

Detects endogenous human BAK in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BAK Antibody

Detection of Human BAK antibody by Western Blot.

Detection of Human BAK by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, 293T human embryonic kidney epithelial cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human BAK Monoclonal Antibody (Catalog # MAB8161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BAK at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
BAK antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

BAK in Jurkat Human Cell Line.

BAK was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human BAK Monoclonal Antibody (Catalog # MAB8161) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BAK Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, 293T human embryonic kidney epithelial cell line, and Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB8161 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAK

BAK (Bcl-2 homologous antagonist/killer; also know as BAK1 and Bcl-2-like protein 7) is a 25‑30 kDa member of the BCL-2 family of proteins. It is widely expressed and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK contains three Bcl-2 homology domains (aa 74‑88, 117‑136 and 169‑184), a Zn-binding region (aa 160‑166) and a C-terminal transmembrane segment (aa 188‑205). Amino acids 67‑94 mediate oligomerization of BAK. There are two potential isoform variants; one shows an alternate start site at Met 96, while a second shows a deletion of aa 46‑66. Over amino acids 20‑124, human BAK shares 76% aa identity with mouse BAK.

Long Name

Bcl-2 Antagonist/Killer

Alternate Names

BAK1, BCL2L7

Entrez Gene IDs

578 (Human); 12018 (Mouse)

Gene Symbol

BAK1

UniProt

Additional BAK Products

Product Documents for Human BAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human BAK Antibody

For research use only

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