Human BAI1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB49691P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 480912

View all BAI1 Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human BAI1
Ala31-Thr879
Accession # O14514

Specificity

Detects human BAI1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BAI1 PE-conjugated Antibody

Detection of BAI1 in HEK293 Human Cell Line Transfected with Human BAI1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human BAI1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human BAI1 PE-conjugated Monoclonal Antibody (Catalog # FAB49691P). Quadrant markers were set based on Mouse IgG1 isotype control antibody staining (IC002P, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human BAI1 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 Human Cell Line Transfected with Human BAI1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BAI1

Brain Angiogenesis Inhibitor 1 (BAI1) is a 170 kDa 7-transmembrane domain G protein-coupled receptor (GPCR) that has a large N-terminal extracellular region with an RGD motif, five thrombospondin type I repeats, and a juxtamembrane GPS (GPCR proteolytic cleavage site) (1). Within the extracellular domain (ECD) up to the GPS (amino acids 31‑879), mature human BAI1 shares 94% amino acid sequence identity with mouse and rat BAI1. BAI1 is preferentially expressed on brain neurons but also is found on astrocytes and macrophages and in the pancreas, stomach, and colon (1‑8). BAI1 can be cleaved within the GPS to release a 120 kDa fragment termed Vasculostatin which corresponds to nearly the entire N-terminal ECD (9). Generation of additional soluble fragments suggests the cleavage of BAI1 at multiple sites (9, 10). BAI1 fragments interact with Integrin alphaV beta5 or CD36 on microvascular endothelial cells to inhibit cell proliferation and migration (10, 11). Overexpression of BAI1 in glioblastoma or pancreatic adenocarcinoma cells inhibits their tumorigenicity and the development of tumor-associated neovascularization (6, 12). Fragments of the ECD, including Vasculostatin, also suppress in vivo angiogenesis and tumor growth (1, 9, 11). BAI1 is down‑regulated in glioblastoma, carcinomas of the pancreas, colon, and stomach and also in experimental ischemia (2, 4, 6‑8). Its expression is inversely correlated with tumor vascularity in colorectal and pulmonary carcinomas (8, 13). On macrophages and astrocytes, BAI1 mediates the phagocytosis of apoptotic cells through recognition of cell surface phosphatidylserine (5).

References

  1. Nishimori, H. et al. (1997) Oncogene 15:2145.
  2. Koh, J.T. et al. (2001) Brain Res. Mol. Brain Res. 87:223.
  3. Mori, K. et al. (2002) Neurosci. Res. 43:69.
  4. Kaur, B. et al. (2003) Am. J. Pathol. 162:19.
  5. Park, D. et al. (2007) Nature 450:430.
  6. Duda, D.G. et al. (2002) Br. J. Cancer 86:490.
  7. Lee, J.H. et al. (2001) Int. J. Oncol. 18:355.
  8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13:967.
  9. Kaur, B. et al. (2005) Oncogene 24:3632.
  10. Koh, J.T. et al. (2004) Exp. Cell Res. 294:172.
  11. Kaur, B. et al. (2009) Cancer Res. 69:1212.
  12. Kang, X. et al. (2006) Cancer Gene Ther. 13:385.
  13. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5:181.

Long Name

Brain-specific Angiogenesis Inhibitor 1

Alternate Names

brain-specific angiogenesis inhibitor 1, FLJ41988, GDAIF

Entrez Gene IDs

575 (Human); 107831 (Mouse); 362931 (Rat)

Gene Symbol

ADGRB1

UniProt

O14514

Additional BAI1 Products

Product Documents for Human BAI1 PE-conjugated Antibody

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Certificate of Analysis

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Product Specific Notices for Human BAI1 PE-conjugated Antibody

For research use only

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