Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, and human skeletal muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Arylsulfatase B/ARSB at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Arylsulfatase B/ARSB in Human Liver.

Arylsulfatase B/ARSB was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Arylsulfatase B/ARSB by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Arylsulfatase B/ARSB at approximately 59 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Arylsulfatase B/ARSB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4415) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Arylsulfatase B/ARSB Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Arylsulfatase B/ARSB

ARSB, also known as N-acetylgalactosamine-4-sulfatase, is a lysosomal glycoprotein and one of at least nine human arylsulfatases that catalyze the hydrolysis of sulfate ester bonds. ARSB deficiency is causative of the lysosomal storage disease Mucopolysaccharidosis VI. Human ARSB is cleaved intracellularly into fragments which remain associated with a disulfide linkage. Mature human ARSB (aa 37-533) shares 80% amino acid sequence identity with mouse and rat ARSB.

Alternate Names

ARSB, ASB, N-acetylgalactosamine-4-sulfatase

Entrez Gene IDs

411 (Human); 11881 (Mouse); 25227 (Rat)

Gene Symbol

ARSB

UniProt

P15848

Additional Arylsulfatase B/ARSB Products

Product Documents for Human Arylsulfatase B/ARSB Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Arylsulfatase B/ARSB Antibody

For research use only

Citations
Reviews

Human Arylsulfatase B/ARSB Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4415

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Arylsulfatase B/ARSB Antibody

Detection of Human Arylsulfatase B/ARSB by Western Blot.