Human APRIL/TNFSF13 Alexa Fluor™ Plus 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF884AFP594

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AF884AFP594-100UG
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Goat IgG

View all APRIL/TNFSF13 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human APRIL/TNFSF13

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

ELISA

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

References

  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
  6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  7. He, B. et al. (2004) J. Immunol. 172:3268.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
  10. Day, E.S. et al. (2005) Biochemistry 44:1919.
  11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat); 102126587 (Cynomolgus Monkey)

Gene Symbol

TNFSF13

Additional APRIL/TNFSF13 Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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