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Human APP/Protease Nexin II Pan Specific Antibody

Bio-Techne includes R&D Systems | Catalog # AF1168

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AF1168
AF1168-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human APP/Protease Nexin II Pan Specific Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human APP 695+1 frameshift mutant
Leu18-Arg336
Accession # NP_958817

Specificity

Detects human APP695+1 and human APP770 in direct ELISAs and Western blots. This antibody recognizes a common epitope present on APP695+1 and APP770 found within the segments Leu18 - Arg288 or Pro365 - Arg411 of APP770 (Accession P05067).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human APP/Protease Nexin II Pan Specific Antibody

APP/Protease Nexin II in Human Brain.

APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's disease cortex) using Goat Anti-Human APP/Protease Nexin II Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1168) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human APP/Protease Nexin II Pan Specific Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Western Blot

0.1 µg/mL
Sample: Recombinant Human APP+1 Western Blot Standard (Catalog # WBC012)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF1168 in the following applications:

Published Applications

Read 3 publications using AF1168 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APP

Amyloid precursor protein (APP) is a type I membrane protein with several human isoforms due to alternative splicing. APP-770, -751, and -733 contain a Kunitz protease inhibitor (KPI) domain (residue 291 - 342) and APP-695 does not. APP is a cell surface molecule with many functions. It can be processed proteolytically in two different pathways. In one pathway, beta- and gamma-secretase cleave at the  beta site between residue 670 and 671 and the gamma site between residue 711 and 714 to produce beta‑amyloid peptide (A beta40 and A beta42), a major component in plaques found in brains of patients with Alzheimer's disease (1). The other pathway involves alpha-secretase that cleaves residues between 687 and 688. It is antiamyloidogenic due to its benign character and the prevention of the A beta peptide formation (2). Soluble APP containing the KPI domain, also referred to as protease nexin II, is a potent inhibitor of serine proteases and may have additional functions. For example, it may regulate the contact face of blood coagulation and limit thrombosis specially in brain due to its localization and coagulation factor XI inhibiting activity (3, 4).

References

  1. Haass, C. (2004) EMBO J. 23:483.
  2. Lichtenthaler, S. F. and C. Haass (2004) J. Clin. Invest. 113:1384.
  3. Badellino, K.O. and P.N. Walsh (2000) Biochemistry 39:4769.
  4. Xu, F. et al. (2005) Proc. Natl. Acad. Sci USA. 102:18135.

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

UniProt

Additional APP Products

Product Documents for Human APP/Protease Nexin II Pan Specific Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human APP/Protease Nexin II Pan Specific Antibody

For research use only

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