Detects human ApoE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ApoA1 or rhApoA2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human Apolipoprotein E/ApoE Antibody

Detection of Human Apolipoprotein E/ApoE by Western Blot.

Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Apolipoprotein E/ApoE Monoclonal Antibody (Catalog # MAB41441) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Apolipoprotein E/ApoE at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Apolipoprotein E/ApoE Antibody

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: Human lung tissue and human ovary tissue

Reviewed Applications

Read 1 review rated 4 using MAB41441 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein E/ApoE

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders. Mature human ApoE shares 71% amino acid sequence identity with mouse and rat ApoE.

Alternate Names

APOE

Entrez Gene IDs

348 (Human); 11816 (Mouse); 25728 (Rat)

Gene Symbol

APOE

UniProt

P02649

Additional Apolipoprotein E/ApoE Products

Product Documents for Human Apolipoprotein E/ApoE Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human Apolipoprotein E/ApoE Antibody

For research use only

Citations
Reviews

Human Apolipoprotein E/ApoE Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB41441

Key Product Details

Species Reactivity

Validated:

Cited:

Applications