Human Apolipoprotein A‑I/ApoA1 Antibody
Catalog # AF3664 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Product Summary for Human Apolipoprotein A‑I/ApoA1 Antibody
Immunogen
Asp25-Gln267
Accession # P02647
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Apolipoprotein A‑I/ApoA1 Antibody
Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Human Apolipoprotein A‑I/ApoA1 by Simple WesternTM.
Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Apolipoprotein A‑I/ApoA1 Antibody
Simple Western
Sample: Human serum
Western Blot
Sample: Human Plasma
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein A-I/ApoA1
Product Documents for Human Apolipoprotein A‑I/ApoA1 Antibody
Product Specific Notices for Human Apolipoprotein A‑I/ApoA1 Antibody
For research use only
Citations for Human Apolipoprotein A‑I/ApoA1 Antibody (4)
Citations are publications that use Bio-Techne products. Selected citations for Human Apolipoprotein A‑I/ApoA1 Antibody include:
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Species: Human
Sample Types: Extracellular
Applications: Western BlotPJ Yao et al. (2021-10-31), Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease Biomedicines, 2021-19(11).
PMID: 34829816 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ImmunoprecipitationU Smole et al. (2020-06-22), Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity Nat. Immunol., 2020-021(7):756-765.
PMID: 32572240 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotH Tadokoro et al. (2020-04-10), Adenosine leakage from perforin-burst extracellular vesicles inhibits perforin secretion by cytotoxic T-lymphocytes PLoS ONE, 2020-015(4):e0231430.
PMID: 32275689 -
Species: Human
Sample Types: Plasma
Applications: Western BlotZhou G et al. (2013-03-18), Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2). J Biol Chem, 2013-0288(17):11940-8.
PMID: 23508960
There are no citations that match your criteria.
Reconstitution Calculator
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