Human Apolipoprotein A‑I/ApoA1 Antibody

Name: Human Apolipoprotein A‑I/ApoA1 Antibody
Brand: R&D Systems
SKU: AF3664
Price: 140 USD

Catalog # AF3664 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human Apolipoprotein A‑I/ApoA1 Antibody

Immunogen

Human Plasma ApoA1 and E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647

Specificity

Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Apolipoprotein A‑I/ApoA1 Antibody

Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Apolipoprotein A‑I/ApoA1 by Simple Western^TM.

Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Apolipoprotein A‑I/ApoA1 Antibody

Application

Recommended Usage

Simple Western

5 µg/mL
Sample: Human serum

Western Blot

0.5 µg/mL
Sample: Human Plasma

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-I/ApoA1

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Entrez Gene IDs

335 (Human); 11806 (Mouse); 25081 (Rat)

Gene Symbol

APOA1

UniProt

P02647

Product Documents for Human Apolipoprotein A‑I/ApoA1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Apolipoprotein A‑I/ApoA1 Antibody

For research use only

Citations for Human Apolipoprotein A‑I/ApoA1 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Apolipoprotein A‑I/ApoA1 Antibody include:

Species: Human
Sample Types: Extracellular
Applications: Western Blot

PJ Yao et al. (2021-10-31), Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease Biomedicines, 2021-19(11).
PMID: 34829816
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation

U Smole et al. (2020-06-22), Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity Nat. Immunol., 2020-021(7):756-765.
PMID: 32572240
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

H Tadokoro et al. (2020-04-10), Adenosine leakage from perforin-burst extracellular vesicles inhibits perforin secretion by cytotoxic T-lymphocytes PLoS ONE, 2020-015(4):e0231430.
PMID: 32275689
Species: Human
Sample Types: Plasma
Applications: Western Blot

Zhou G et al. (2013-03-18), Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2). J Biol Chem, 2013-0288(17):11940-8.
PMID: 23508960

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