Human Aminopeptidase PILS/ARTS1 Antibody

Name: Human Aminopeptidase PILS/ARTS1 Antibody
Brand: R&D Systems
SKU: AF2334

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2334

Citations (4)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase PILS/ARTS1 Ala37-Met941 Accession # EAW96077
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse ARTS1 is observed.

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Applications for Human Aminopeptidase PILS/ARTS1 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	COLO 205 human colorectal adenocarcinoma cells and HDLM‑2 human Hodgkin's lymphoma cells
Immunohistochemistry	1-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human Aminopeptidase PILS/ARTS1 (Catalog # 2334-ZN), see our available Western blot detection antibodies
Immunocytochemistry	1-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Aminopeptidase PILS/ARTS1 Antibody

	Western Blot Detection of Human Aminopeptidase PILS/ARTS1 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HDLM-2 human Hodgkin's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Aminopeptidase PILS/ARTS1 in A549 Human Cell Line. Aminopeptidase PILS/ARTS1 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry Aminopeptidase PILS/ARTS1 in Human Liver. Aminopeptidase PILS/ARTS1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Simple Western Detection of Human Aminopeptidase PILS/ARTS1 by Simple Western^TM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase PILS/ARTS1 at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Aminopeptidase PILS/ARTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2334). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Aminopeptidase PILS/ARTS1

The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AiminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2‑4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2‑5).

Item	Value
References	Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego. Cui, X. et al. (2002) J. Clin. Invest. 110:515. York, I.A. et al. (2002) Nat. Immunol. 3:1177. Serwold, T. et al. (2002) Nature 419:480. Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Long Name	Aminopeptidase Puromycin-insensitive Leucyl-specific
Entrez Gene IDs	51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)
Alternate Names	A-LAP, A-LAPALAP, ARTS-1PILSAP, ARTS1, ARTS1aminopeptidase regulator of TNFR1 shedding, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, EC 3.4.11, EC 3.4.11.-, EC 3.4.11.1, ERAAP1, ERAP1, KIAA0525APPILS, PILS-AP, PILS-APERAAP, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator, endoplasmic reticulum aminopeptidase 1, endoplasmic reticulum aminopeptidase associated with antigen processing

Citations for Human Aminopeptidase PILS/ARTS1 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human Aminopeptidase PILS/ARTS1 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

I Temponeras et al. (2022), ERAP2 Inhibition Induces Cell-Surface Presentation by MOLT-4 Leukemia Cancer Cells of Many Novel and Potentially Antigenic Peptides International Journal of Molecular Sciences, 23(3).
PMID: 35163832
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

P Jiang et al. (2021), Interaction between ERAP Alleles and HLA Class I Types Support a Role of Antigen Presentation in Hodgkin Lymphoma Development Cancers, 13(3).
PMID: 33499248
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A Papakyriak et al. (2018), The partial dissociation of MHC class I bound peptides exposes their N terminus to trimming by endoplasmic reticulum aminopeptidase 1 J. Biol. Chem., 0(0).
PMID: 29599287
Species: Human
Sample Types: Whole Cells
Applications: ICC

SR Harrison et al. (2018), Inositol-Requiring Enzyme 1-Mediated Downregulation of MicroRNA (miR)-146a and miR-155 in Primary Dermal Fibroblasts across ThreeTNFRSF1AMutations Results in Hyperresponsiveness to Lipopolysaccharide Front Immunol, 9(0):173.
PMID: 29467762

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Human Aminopeptidase PILS/ARTS1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Aminopeptidase PILS/ARTS1 Antibody

Scientific Data Examples for Human Aminopeptidase PILS/ARTS1 Antibody

Western Blot

Immunocytochemistry

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Aminopeptidase PILS/ARTS1

Citations for Human Aminopeptidase PILS/ARTS1 Antibody (4)

Product Documents for Human Aminopeptidase PILS/ARTS1 Antibody

Product Datasheets

COA

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