Detects human Alkaline Phosphatase/ALPP in direct ELISAs. In direct ELISAs, this antibody cross-reacts at 100% with recombinant human (rh) ALPPL2, but no cross-reactivity with rhAlkaline Phosphatase/ALPI is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Alkaline Phosphatase/ALPP Antibody

Detection of Human Alkaline Phosphatase/ALPP by Western Blot.

Western blot shows lysates of JAR human choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # MAB59051) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Alkaline Phosphatase/ALPP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Alkaline Phosphatase/ALPP in Human Placenta.

Alkaline Phosphatase/ALPP was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # MAB59051) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Alkaline Phosphatase/ALPP by Simple Western^TM.

Simple Western lane view shows lysates of JAR human choriocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Alkaline Phosphatase/ALPP at approximately 74 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # MAB59051). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Alkaline Phosphatase/ALPP Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Simple Western

10 µg/mL
Sample: JAR human choriocarcinoma cell line

Western Blot

1 µg/mL
Sample: JAR human choriocarcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB59051 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPP

Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPP gene encodes the 67 kDa placental isozyme, also known as PLAP. In comparison, ALPI, ALPL, and ALPPL2 encode intestinal, liver/bone/kidney, and placental-like or germ cell APs, respectively. The placental alkaline phosphatase is a glycosyl phosphatidylinositol (GPI) anchored protein that is expressed on the cell surface as a homodimer. Human APs are ectopically expressed in several cancers, and their serum levels are useful tumor markers. Mature human Alkaline Phosphatase/ALPP shares 74% amino acid sequence homology with the mouse and rat enzymes.

Long Name

Alkaline Phosphatase, Placental Type

Alternate Names

Alkaline Phosphatase, PLAP, SEAP

Entrez Gene IDs

250 (Human); 11650 (Mouse); 367308 (Rat)

Gene Symbol

ALPP

UniProt

P05187

Additional Alkaline Phosphatase/ALPP Products

Product Documents for Human Alkaline Phosphatase/ALPP Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Alkaline Phosphatase/ALPP Antibody

For research use only

Citations
Reviews

Human Alkaline Phosphatase/ALPP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB59051

Key Product Details

Species Reactivity

Validated:

Cited:

Applications