Western blot shows lysates of PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Ajuba Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5594) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ajuba at approximately 55 to 60 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human Ajuba Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: PC-3 human prostate cancer cell line, A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ajuba

Ajuba (an Urdu word meaning "curiosity") is a 55‑60 kDa member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha-catenin:F-actin binding, beta-catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF-kappa B activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538 amino acids (aa) in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa 1‑335), an NES (aa 280‑287), and three LIM domains that bind multiple proteins (aa 336‑397, 401‑461 and 462‑530).

Alternate Names

JUB

Entrez Gene IDs

84962 (Human); 16475 (Mouse); 85265 (Rat)

Gene Symbol

AJUBA

UniProt

Q96IF1

Additional Ajuba Products

Product Documents for Human Ajuba Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Ajuba Antibody

For research use only

Citations
Reviews

Human Ajuba Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5594

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Ajuba Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Ajuba Antibody

Detection of Human Ajuba by Western Blot.