Human AHR Antibody

Name: Human AHR Antibody
Brand: rnd
SKU: AF6185

Catalog # AF6185

Datasheet / COA / SDS

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Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human AHR Asn704-Leu848 Accession # P35869
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Sheep IgG
Specificity:	Detects human AHR in Western blots.

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Applications

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Item	Value
Knockout Validated	AHR is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in AHR knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human AHR Antibody Scientific Data Examples

	Western Blot Detection of Human AHR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry AHR in HeLa Human Cell Line. AHR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Intracellular Staining by Flow Cytometry Detection of AHR in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
	Simple Western Detection of Human AHR by Simple Western^TM. Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for AHR at approximately 109 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Knockout Validated Western Blot Shows Human AHR Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and AHR knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AHR

AHR (Aryl-hydrocarbon receptor; also known as bHLHE76) is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a down‑regulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848 amino acids (aa) in length. It contains a 10 aa prosegment, plus a 838 aa mature molecule that contains a DNA binding motif (aa 13-40), a bHLH region (aa 41-81), and two PAS domains (aa 111-342). Over aa 704-848, human AHR shares 70% aa identity with mouse AHR.

Item	Value
Long Name	Aryl Hydrocarbon Receptor
Entrez Gene IDs	196 (Human); 11622 (Mouse); 25690 (Rat)
Alternate Names	AH-receptor, AHR, Ah receptor, BHLHE76, Class E basic helix-loop-helix protein 76, aryl hydrocarbon receptor, bHLHe76aromatic hydrocarbon receptor

Human AHR Antibody

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Key Product Details

Complete Your Research

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Applications

Human AHR Antibody Scientific Data Examples

Western Blot

Immunocytochemistry

Intracellular Staining by Flow Cytometry

Simple Western

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: AHR

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Product Documents

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COA

Certificate of Analysis

SDS

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