Human ADP-Sugar Pyrophosphatase/NUDT5 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6414AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 739044
Product Specifications
Immunogen
E. coli-derived recombinant human ADP-Sugar Pyrophosphatase/NUDT5
Specificity
Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ADP-Sugar Pyrophosphatase/NUDT5
ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).
References
- McLennan, A.G. (2006) Cell Mol. Life Sci. 63:123.
- Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
- Zha, M. et al. (2008) J. Mol. Biol. 379:568.
- Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
- Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
- Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.
Alternate Names
Nudix motif 5, YSA1, YSA1H
Entrez Gene IDs
11164 (Human)
Gene Symbol
NUDT5
UniProt
Additional ADP-Sugar Pyrophosphatase/NUDT5 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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