Is ACE-1 the same as ACE?

Name: Human ACE/CD143 Antibody
Brand: R&D Systems
SKU: AF929

Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

Human ACE/CD143 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF929

Citations (4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human ACE/CD143 Leu30-Leu1261 Accession # P12821
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects ACE/CD143 in direct ELISAs and Western blots.

View Full Technical Details for Human ACE/CD143 Antibody

Applications for Human ACE/CD143 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human ACE/CD143 Somatic Form (Catalog # 929-ZN)
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human ACE/CD143 Antibody

	Flow Cytometry Detection of ACE/CD143 in Human Mature Monocyte-derived Dendritic Cells by Flow Cytometry. Human mature monocyte-derived dendritic cells were stained with Goat Anti-Human ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF929, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
	Immunohistochemistry ACE/CD143 in Human Kidney. ACE/CD143 was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF929) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry ACE/CD143 in Human Kidney. ACE/CD143 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF929) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ACE/CD143

ACE (also known as peptidyl-dipetidase A) is a zinc metallopeptidase important for blood pressure control and water and salt metabolism (2). It cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II and inactivates bradykinin by the sequential removal of two C-terminal dipeptides. In addition to the two physiological substrates, ACE cleaves C-terminal dipeptides from various oligopeptides with a free C-terminus. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid beta -peptide (A beta ), retards A beta aggregation, deposition, fibril formation, and inhibits cytotoxicity (3).

ACE is a type I membrane protein and exists in two isoforms (2). Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar domains called N- and C-domains, each of which contains the HExxH consensus sequence for zinc binding. Germinal ACE, found exclusively in the testes, comprises a single catalytically active domain identical to the C-domain of somatic ACE except for an N-terminal 67 residue germinal ACE-specific sequence. Physiological functions of the two tissue-specific isozymes are not interchangeable (4). For example, sperm-specific expression of the germinal ACE, not the somatic ACE, in ACE knockout male mice restored fertility.

Soluble ACE is present in many biological fluids, such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid (2). The soluble ACE is derived from the membrane forms by actions of secretases or sheddases. The identities of the secretases have not been revealed, although they belong to the family of zinc metallopeptidases (5, 6).

Item	Value
References	Soubrier, et al. (1988) Proc. Natl. Acad. Sci. USA 85:9386. Corvol, P. and T.A. Williams (1998) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds): San Diego, Academic Press, p. 1066. Hu, et al. (2001) J. Biol. Chem. 276:47863. Kessler, et al. (2000) J. Biol. Chem. 275:26259. Eyries, et al. (2001) J. Biol. Chem. 276:5525. Alfalah, et al. (2001) J. Biol. Chem. 276:21105.
Long Name	Angiotensin I Converting Enzyme
Entrez Gene IDs	1636 (Human); 11421 (Mouse)
Alternate Names	ACE, ACE1angiotensin converting enzyme, somatic isoform, CD143, CD143 antigen, DCP, DCP1, DCP1angiotensin-converting enzyme, Dipeptidyl carboxypeptidase I, EC 3.2.1.-, EC 3.4.15.1, Kininase II, MGC26566, MVCD3, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, carboxycathepsin, dipeptidyl carboxypeptidase 1, peptidase P, testicular ECA

Citations for Human ACE/CD143 Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human ACE/CD143 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

K Wilkus et al. (2021), Distinctive Properties of Endothelial Cells from Tumor and Normal Tissue in Human Breast Cancer International Journal of Molecular Sciences, 22(16).
PMID: 34445568
Riordan D et al. (2015), Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 10(7):e0128975.
PMID: 26176839
Species: Human
Sample Types: Serum
Applications: Western Blot

Fagyas M et al. (2014), New perspectives in the renin-angiotensin-aldosterone system (RAAS) II: albumin suppresses angiotensin converting enzyme (ACE) activity in human. PLoS ONE, 9(4):e87844.
PMID: 24691203
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Neutralization

Rockx B et al. (2007), Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice. J. Virol., 81(14):7410-23.
PMID: 17507479

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Showing 1 - 4 of 4 citations Showing All

FAQs for Human ACE/CD143 Antibody

Is ACE-1 the same as ACE?

Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

Human ACE/CD143 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human ACE/CD143 Antibody

Scientific Data Examples for Human ACE/CD143 Antibody

Flow Cytometry

Immunohistochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: ACE/CD143

Citations for Human ACE/CD143 Antibody (4)

FAQs for Human ACE/CD143 Antibody

Product Documents for Human ACE/CD143 Antibody

Product Datasheets

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