Human A33 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11365
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2963D
Product Specifications
Immunogen
E. coli-derived recombinant human A33
Ile22-Val235
Accession # Q99795
Ile22-Val235
Accession # Q99795
Specificity
Detects human A33 in direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human A33 Antibody
Detection of Human A33 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB11365) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of A33 in HEK293 cells transfected with A33 vs irrelevant transfectant cells by Flow Cytometry.
HEK293 cells transfected with A33 (A) vs irrelevant transfectant (B) cells were stained with Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB11365) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). View our protocol for Staining Membrane-associated Proteins. View our protocol for Staining Membrane-associated Proteins.Applications for Human A33 Antibody
Application
Recommended Usage
Flow Cytometry
0.10 µg/106 cells
Sample: HEK293 cells transfected with A33 vs irrelevant transfectant cells
Sample: HEK293 cells transfected with A33 vs irrelevant transfectant cells
Western Blot
1 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line
Sample: COLO 205 human colorectal adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: A33
Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Long Name
Cell Surface A33 Antigen
Alternate Names
GPA33
Gene Symbol
GPA33
UniProt
Additional A33 Products
Product Documents for Human A33 Antibody
Product Specific Notices for Human A33 Antibody
For research use only
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