Human 4-1BB Ligand/TNFSF9 Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB22952AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2357A
Product Specifications
Immunogen
E. coli-derived human 4-1BB Ligand/TNFSF9
Specificity
Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: 4-1BB Ligand/TNFSF9
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).
References
- Wang, C. et al. (2009) Immunol. Rev. 229:192.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
- DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
- Langstein, J. et al. (1998) J. Immunol. 160:2488.
- Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
- Baessler, T. et al. (2010) Blood 115:3058.
- Gullo, C. et al. (2010) PLoS One 5:e10845.
- Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
- Salih, H.R. et al. (2001) J. Immunol. 167:4059.
- Wen, T. et al. (2002) J. Immunol. 168:4897.
- Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
- Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
- Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
- Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.
Alternate Names
41BB Ligand, CD137L, TNFSF9
Gene Symbol
TNFSF9
UniProt
Additional 4-1BB Ligand/TNFSF9 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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