Human 4-1BB Ligand/TNFSF9 Alexa Fluor® 647-conjugated Antibody

Name: Human 4-1BB Ligand/TNFSF9 Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems
SKU: FAB22952R

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB22952R

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Recombinant Monoclonal Antibody.

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Species Reactivity:	Human
Immunogen:	E. coli-derived human 4-1BB Ligand/TNFSF9 Arg71-Glu254 Accession # P41273.1
Applications:	Flow Cytometry
Label:	Alexa Fluor 647 (Excitation=650 nm, Emission=668 nm)
Source:	Recombinant Monoclonal Rabbit IgG Clone # 2357A
Specificity:	Detects human 4-1BB Ligand/TNFSF9 in direct ELISA.

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Applications

	Recommended Concentration	Sample
Flow Cytometry	0.25-1 µg/10⁶ cells	Human Daudi cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The 38 kDa human 4-1BB Ligand consists of an 25 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, human 4-1BB Ligand shares 34% aa sequence identity with mouse and rat 4-1BB Ligand. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10).
4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4⁺ and CD8⁺ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through 4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

Item	Value
References	Wang, C. et al. (2009) Immunol. Rev. 229:192. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. Reali, C. et al. (2003) J. Neurosci. Res. 74:67. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551. Langstein, J. et al. (1998) J. Immunol. 160:2488. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601. Baessler, T. et al. (2010) Blood 115:3058. Gullo, C. et al. (2010) PLoS One 5:e10845. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917. Salih, H.R. et al. (2001) J. Immunol. 167:4059. Wen, T. et al. (2002) J. Immunol. 168:4897. Cannons, J.L. et al. (2001) J. Immunol. 167:1313. Bertram, E.M. et al. (2002) J. Immunol. 168:3777. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.
Entrez Gene IDs	8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)
Alternate Names	4-1BB Ligand, 4-1BB-L, 4-1BBL, 41BB Ligand, CD137L, TNFSF9, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand superfamily member 9

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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Human 4-1BB Ligand/TNFSF9 Alexa Fluor® 647-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Formulation, Preparation and Storage

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Background: 4-1BB Ligand/TNFSF9

Product Specific Notices

Citations for Human 4-1BB Ligand/TNFSF9 Alexa Fluor® 647-conjugated Antibody

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