Human 20S Proteasome Antibody

Name: Human 20S Proteasome Antibody
Brand: R&D Systems
SKU: MAB9554
Rating: 1 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB9554

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 863437

View all 20S Proteasome Antibodies »

Product Specifications

Immunogen

Human 20S Proteasome purified from human erthrocytes

Specificity

Detects human 20S Proteasome in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human 20S Proteasome Antibody

Detection of Human 20S Proteasome by Western Blot.

Western blot shows Human 20S Proteasome (Catalog # E-360), Human 20S Immunoproteasome (Catalog # E-370), and Recombinant Human PSMA1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human 20S Proteasome Monoclonal Antibody (Catalog # MAB9554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 20S Proteasome at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

20S Proteasome in A549 Human Cell Line.

20S Proteasome was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human 20S Proteasome Monoclonal Antibody (Catalog # MAB9554) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human 20S Proteasome Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line

Western Blot

2 µg/mL
Sample: Human 20S Proteasome (Catalog # E-360), Human 20S Immunoproteasome (Catalog # E-370), and Recombinant Human PSMA1

Reviewed Applications

Read 1 review rated 1 using MAB9554 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 20S Proteasome

The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings. However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1. The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes. 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.