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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 921215

Product Specifications

Immunogen

E. coli-derived recombinant human 11β‑HSD2
Met105-Arg405
Accession # P80365

Specificity

Detects human 11 beta‑HSD2 in direct ELISA and Western Blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human 11 beta-HSD2 Alexa Fluor® 532-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 11 beta-HSD2

The human enzyme HSD-2/11 beta-HSD2 is encoded by the HSD11B2 gene and codes for a 405 aa protein, with high expression in kidney, colon, pancreas and placenta1. HSD-2 modulates intracellular glucocorticoid levels by catalyzing the conversion of cortisol to the inactive metabolite cortisone2. HSD-2 is abundantly expressed in human placenta and controls fetal glucocorticoid levels to protect the fetus from glucocorticoid exposure. Similarly, HSD-2 expression in the distal nephron protects mineralcorticoid receptors from glucocorticoids and helps regulate blood pressure3. Exposure to major environmental pollutant like Cadmium, inhibits activity of HSD-2 which affects the synthesis of glucocorticoids in placenta and thus contribute to developing preeclamptic conditions.

Long Name

11 beta Hydroxysteroid Dehydrogenase 2

Alternate Names

11 betaHSD2, HSD11B2

Entrez Gene IDs

3291 (Human); 15484 (Mouse); 25117 (Rat)

Gene Symbol

HSD11B2

UniProt

Additional 11 beta-HSD2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human 11 beta-HSD2 Alexa Fluor® 532-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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