HR6A/UBE2A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-553
![Western Blot: HR6A/UBE2A Antibody [NB100-553]](https://resources.bio-techne.com/images/products/HR6A-UBE2A-Antibody-Western-Blot-NB100-553-img0019.jpg "Western Blot: HR6A/UBE2A Antibody [NB100-553]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide which maps to a region between the N-terminus of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319).
Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react with D. melanogaster.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit HR6A/UBE2A Antibody - BSA Free (NB100-553) is a polyclonal antibody validated for use in IHC and WB. Anti-HR6A/UBE2A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HR6A/UBE2A Antibody - BSA Free
Western Blot: HR6A/UBE2A Antibody [NB100-553]
Western Blot: HR6A/UBE2A Antibody [NB100-553] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody NB100-553 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: HR6A/UBE2A Antibody [NB100-553]](https://resources.bio-techne.com/images/products/HR6A-UBE2A-Antibody-Immunohistochemistry-NB100-553-img0016.jpg "Immunohistochemistry-Paraffin: HR6A/UBE2A Antibody [NB100-553]")
Immunohistochemistry-Paraffin: HR6A/UBE2A Antibody [NB100-553]
Immunohistochemistry-Paraffin: HR6A/UBE2A Antibody [NB100-553] - Section of human stomach carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Rad6 used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for HR6A/UBE2A Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 -1:2000
Immunohistochemistry-Paraffin
1:500 -1:2000
Western Blot
1:5000-1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HR6A/UBE2A
Long Name
Ubiquitin-conjugating Enzyme E2A
Alternate Names
RAD6A, UBE2A
Entrez Gene IDs
7319 (Human)
Gene Symbol
UBE2A
UniProt
Additional HR6A/UBE2A Products
Product Documents for HR6A/UBE2A Antibody - BSA Free
Product Specific Notices for HR6A/UBE2A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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