Western blot shows lysates of recombinant HIV-1 Gag p24. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti- HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIV-1 Gag p24 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Viral HIV‑1 Gag p24 ELISA Standard Curve.

Recombinant Viral HIV-1 Gag p24 protein was serially diluted 2-fold and captured by Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB73602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for HIV-1 Gag p24 Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral HIV‑1 Gag p24 Monoclonal Antibody (Catalog # MAB73602).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the HIV-1 Gag p24 DuoSet ELISA Kit (Catalog # DY7360-05) for convenient development of a sandwich ELISA or the HIV-1 Gag p24 Quantikine ELISA Kit (Catalog # DHP240) for a complete optimized ELISA.

Western Blot

0.1 µg/mL
Sample: Recombinant HIV-1 Gag p24

Reviewed Applications

Read 1 review rated 5 using MAB73601 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Entrez Gene IDs

155030 (Viral)

Gene Symbol

GAG

UniProt

Q77YG1_9HIV1

Additional HIV-1 Gag p24 Products

Product Documents for HIV-1 Gag p24 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for HIV-1 Gag p24 Antibody

For research use only

Citations
Reviews

HIV-1 Gag p24 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB73601

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for HIV-1 Gag p24 Antibody

Detection of HIV‑1 Gag p24 by Western Blot.