His Tag PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC0501P
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Multi-Species
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # AD1.1.10R
Product Specifications
Immunogen
His-tagged peptide
Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags located at the amino- or carboxyl- terminus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for His Tag PE-conjugated Antibody
Detection of protein binding with Mouse anti-His Tag PE in HEK293 cells by Flow Cytometry
In a functional flow cytometry test, recombinant SARS-CoV2 S1 RBD His-tagged protein (50 ng/mL, 10500-CV) binds to HEK293 cells transfected with Human ACE-2 and eGFP. (A) Protein binding was detected with Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) or Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) alone. View our protocol for View our protocol for Staining Membrane-associated Proteins.Applications for His Tag PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HEK293 cells transfected with Human ACE-2 and eGFP.
Sample: HEK293 cells transfected with Human ACE-2 and eGFP.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: His Tag
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag PE-conjugated Antibody
Product Specific Notices for His Tag PE-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...