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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2A Clone # 584308

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1
Pro38-Trp411
Accession # O60243

Specificity

Detects human Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images

Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Antibody (584308) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1

HS6ST1 (Heparan-sulphate 6-O-sulfotransferase 1) is a single-pass type II transmembrane protein that catalyses the transfer of sulphate from PAPS (3'-phosphoadenosine 5'-phosphosulphate) to position 6 of the N-sulphoglucosamine residue of heparan sulphate. It is expressed in foetal brain and liver and is thought to play a significant role in axonal guidance through sulphation of heparan sulphate proteoglycans, with HS6ST1 expression mirroring that of Slit1 and Slit2.

Alternate Names

DKFZp547H098, EC 2.8.2, EC 2.8.2.-, FLJ25392, heparan sulfate 6-O-sulfotransferase 1, heparan-sulfate 6-sulfotransferase, HS6ST-1, HS6STheparan-sulfate 6-O-sulfotransferase 1, MGC116899, MGC116901

Gene Symbol

HS6ST1

Additional Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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