Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25943
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # ARC53251
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein of human GPX1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) (NBP3-25943) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] -
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of various lysates, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] -
Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of Rat liver, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] -
Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutathione Peroxidase 1/GPX1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.Applications for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry
1:50 - 200
Immunohistochemistry-Paraffin
1:50 - 200
Western Blot
1:2000 - 1:12000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Glutathione Peroxidase 1/GPX1
Alternate Names
CGPx, GPX1, GSHPX1
Gene Symbol
GPX1
Additional Glutathione Peroxidase 1/GPX1 Products
Product Documents for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)
Product Specific Notices for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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