Glucagon Antibody (53) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05184

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NBP1-05184
NBP1-05184-0.025mg
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Conjugate
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Alexa Fluor 350
NBP1-05184AF350
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Alexa Fluor 405
NBP1-05184AF405
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Alexa Fluor 488
NBP1-05184AF488
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Alexa Fluor 532
NBP1-05184AF532
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Alexa Fluor 594
NBP1-05184AF594
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Alexa Fluor 647
NBP1-05184AF647
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Alexa Fluor 700
NBP1-05184AF700
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Alexa Fluor 750
NBP1-05184AF750
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Alexa Fluor Plus 405
NBP1-05184AFP405
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Alexa Fluor Plus 488
NBP1-05184AFP488
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Alexa Fluor Plus 555
NBP1-05184AFP555
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Alexa Fluor Plus 594
NBP1-05184AFP594
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Alexa Fluor Plus 647
NBP1-05184AFP647
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Alexa Fluor Plus 680
NBP1-05184AFP680
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Allophycocyanin
NBP1-05184APC
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Biotin
NBP1-05184B
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CoraFluor 1
NBP1-05184CL1
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DyLight 350
NBP1-05184UV
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DyLight 405
NBP1-05184V
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DyLight 488
NBP1-05184G
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DyLight 550
NBP1-05184R
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DyLight 594
NBP1-05184DL594
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DyLight 650
NBP1-05184C
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DyLight 680
NBP1-05184FR
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DyLight 755
NBP1-05184IR
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FITC
NBP1-05184F
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HRP
NBP1-05184H
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Janelia Fluor 525
NBP1-05184JF525
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Janelia Fluor 549
NBP1-05184JF549
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Janelia Fluor 646
NBP1-05184JF646
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Janelia Fluor 669
NBP1-05184JF669
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mFluor Violet 450 SE
NBP1-05184MFV450
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mFluor Violet 500 SE
NBP1-05184MFV500
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mFluor Violet 610 SE
NBP1-05184MFV610
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PE
NBP1-05184PE
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PerCP
NBP1-05184PCP
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Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 53

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glucagon Antibodies »

Product Specifications

Immunogen

Synthetic GLP-1(9-18)

Epitope

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Specificity

Based on inhibition ELISA, It cross-reacts <2% with GLP-1(7-36)amide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for Glucagon Antibody (53) - BSA Free

Glucagon Antibody (53) - BSA Free [NBP1-05184] - BSA Free [NBP1-05184]

Applications for Glucagon Antibody (53) - BSA Free

Application
Recommended Usage

ELISA

1:6000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05184 binds free GLP-1(9-36)amide in solution. NBP1-05184 has higher affinity for GLP-1(9-36)amide than NBP2-23591 as determined by inhibition. WB: NBP1-05184 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glucagon

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (53) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucagon Antibody (53) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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