Glucagon Antibody (09) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21803
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) - BSA Free [NBP2-21803]](https://resources.bio-techne.com/images/products/Glucagon-Antibody-09-Immunocytochemistry-Immunofluorescence-NBP2-21803-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) - BSA Free [NBP2-21803]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Human, Mouse, Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Sandwich ELISA, Sandwich ELISA Capture, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 09
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic full length oxyntomodulin coupled to carrier.
Epitope
The epitope lies within the C-terminal region of glucagon.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:32953935).
Specificity
Binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Concentration for any given batch will be between 0.85 - 1.15 mg/mL.
Scientific Data Images for Glucagon Antibody (09) - BSA Free
Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) - BSA Free [NBP2-21803]
Immunocytochemistry/Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000![Immunohistochemistry-Paraffin: Glucagon Antibody (09) - BSA Free [NBP2-21803]](https://resources.bio-techne.com/images/products/Glucagon-Antibody-09-Immunohistochemistry-Paraffin-NBP2-21803-img0002.jpg "Immunohistochemistry-Paraffin: Glucagon Antibody (09) - BSA Free [NBP2-21803]")
Immunohistochemistry-Paraffin: Glucagon Antibody (09) - BSA Free [NBP2-21803]
Immunohistochemistry-Paraffin: Glucagon Antibody (09) [NBP2-21803] - Staining of human pancreas tissue . Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000
Sandwich ELISA: Glucagon Antibody (09) - BSA Free [NBP2-21803] - Glucagon Antibody (09) [NBP2-21803] - The calibration curve of a sandwich assay for Glucagon using NBP2-21803 as the capture antibody and NBP2-21802B as the biotinylated detection antibody.
Applications for Glucagon Antibody (09) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:4000
Immunohistochemistry
1:8000
Immunohistochemistry-Paraffin
1:8000
Sandwich ELISA
1:100-1:2000
Application Notes
Use in Flow reported in scientific literature (PMID:33284818). ELISA: NBP2-21803 binds free glucagon in solution. NBP2-21803 when used as capture antibody forms a sandwich ELISA pair with NBP2-21802B as biotinylated detection antibody for measuring glucagon. IHC: NBP2-21803 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:8000. IF: NBP2-21803 was used in IF with a dilution of 1:4000.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4), 0.5M NaCl
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Glucagon
Alternate Names
GCG, GRPP
Gene Symbol
GCG
Additional Glucagon Products
Product Documents for Glucagon Antibody (09) - BSA Free
Product Specific Notices for Glucagon Antibody (09) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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