Ghrelin/Obestatin Antibody (45) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47183

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NBP1-47183-0.1mg
NBP1-47183-0.025mg
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Conjugate
Catalog #
Alexa Fluor 350
NBP1-47183AF350
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Alexa Fluor 405
NBP1-47183AF405
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Alexa Fluor 488
NBP1-47183AF488
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Alexa Fluor 532
NBP1-47183AF532
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Alexa Fluor 594
NBP1-47183AF594
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Alexa Fluor 647
NBP1-47183AF647
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Alexa Fluor 700
NBP1-47183AF700
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Alexa Fluor 750
NBP1-47183AF750
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Alexa Fluor Plus 405
NBP1-47183AFP405
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Alexa Fluor Plus 488
NBP1-47183AFP488
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Alexa Fluor Plus 555
NBP1-47183AFP555
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Alexa Fluor Plus 594
NBP1-47183AFP594
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Alexa Fluor Plus 647
NBP1-47183AFP647
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Alexa Fluor Plus 680
NBP1-47183AFP680
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Allophycocyanin
NBP1-47183APC
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Biotin
NBP1-47183B
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CoraFluor 1
NBP1-47183CL1
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DyLight 350
NBP1-47183UV
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DyLight 405
NBP1-47183V
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DyLight 488
NBP1-47183G
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DyLight 550
NBP1-47183R
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DyLight 594
NBP1-47183DL594
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DyLight 650
NBP1-47183C
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DyLight 680
NBP1-47183FR
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DyLight 755
NBP1-47183IR
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FITC
NBP1-47183F
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HRP
NBP1-47183H
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Janelia Fluor 525
NBP1-47183JF525
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Janelia Fluor 549
NBP1-47183JF549
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Janelia Fluor 646
NBP1-47183JF646
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Janelia Fluor 669
NBP1-47183JF669
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mFluor Violet 450 SE
NBP1-47183MFV450
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mFluor Violet 500 SE
NBP1-47183MFV500
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mFluor Violet 610 SE
NBP1-47183MFV610
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PE
NBP1-47183PE
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PerCP
NBP1-47183PCP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 45

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ghrelin/Obestatin Antibodies »

Product Specifications

Immunogen

Synthetic human Ser3-octanoyl ghrelin-28

Epitope

Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin

Specificity

Binds human octanoylated ghrelin and human des-acyl ghrelin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for Ghrelin/Obestatin Antibody (45) - BSA Free

ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183]

ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183]

ELISA: Ghrelin/Obestatin Antibody (45) - BSA Free [NBP1-47183] - The calibration curve of a sandwich assay for Ghrelin using NBP1-47183 as the capture antibody and NBP1-47182B as the biotinylated detection antibody.

Applications for Ghrelin/Obestatin Antibody (45) - BSA Free

Application
Recommended Usage

ELISA

1:4000
Application Notes
ELISA: NBP1-47183 binds human ghrelin in solution. NBP1-47183 (as a capture antibody) forms a pair with NBP1-47182 as a biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA. WB: NBP1-47183 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Ghrelin/Obestatin

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons1,2. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form."Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin3.

Alternate Names

appetite-regulating hormone, ghrelin, ghrelin/obestatin preprohormone, ghrelin/obestatin prepropeptide, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRPgrowth hormone secretagogue receptor ligand, obestatin, Protein M46

Gene Symbol

GHRL

Additional Ghrelin/Obestatin Products

Product Documents for Ghrelin/Obestatin Antibody (45) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ghrelin/Obestatin Antibody (45) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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