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Geminin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16639

Novus Biologicals, part of Bio-Techne
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NBP2-16639

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length human Geminin Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Geminin Antibody - BSA Free (NBP2-16639) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Geminin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Geminin Antibody [NBP2-16639]

Immunocytochemistry/ Immunofluorescence: Geminin Antibody [NBP2-16639]

Immunocytochemistry/Immunofluorescence: Geminin Antibody [NBP2-16639] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-16639]

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-16639]

Immunohistochemistry-Paraffin: Geminin Antibody [NBP2-16639] - Detects Geminin protein at nucleus on breast carcinoma.
Immunoprecipitation: Geminin Antibody [NBP2-16639]

Immunoprecipitation: Geminin Antibody [NBP2-16639]

Immunoprecipitation: Geminin Antibody [NBP2-16639] - HCT-116 whole cell extracts using 5 ug of Geminin antibody. Western blot analysis was performed using Geminin antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for Geminin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Blood, separated by Size

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Geminin

Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Gene Symbol

GMNN

Additional Geminin Products

Product Documents for Geminin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Geminin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.