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GATA-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16612

Novus Biologicals, part of Bio-Techne
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NBP2-16612

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%), Porcine (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GATA-2. The exact sequence is proprietary.

Reactivity Notes

Chicken (86%), Xenopus laevis (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GATA-2 Antibody - BSA Free (NBP2-16612) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GATA-2 Antibody - BSA Free

GATA-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GATA-2 Antibody [NBP2-16612] -

GATA2 antibody detects GATA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GATA2 stained by GATA2 antibody (NBP2-16612) diluted at 1:500.
Blue: Fluoroshield with DAPI .
GATA-2 Antibody - BSA Free

Immunohistochemistry-Frozen: GATA-2 Antibody [NBP2-16612] -

GATA2 antibody detects GATA2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: GATA2 stained by GATA2 antibody (NBP2-16612) diluted at 1:250.
Blue: Fluoroshield with DAPI .
GATA-2 Antibody - BSA Free

Western Blot: GATA-2 Antibody [NBP2-16612] -

293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GATA2 antibody (NBP2-16612) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GATA-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GATA-2

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.

Alternate Names

GATA2

Gene Symbol

GATA2

UniProt

Additional GATA-2 Products

Product Documents for GATA-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GATA-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.