FIH-1/HIF-1AN Antibody (23GB6385)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51394
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB6385
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human FIH-1/HIF-1AN (Uniprot: Q9NWT6)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FIH-1/HIF-1AN Antibody (23GB6385)
Immunocytochemistry/ Immunofluorescence: FIH-1/HIF-1AN Antibody (23GB6385)[NBP3-51394] -
Immunocytochemical staining of HeLa cells with FIH-1/HIF-1AN antibody (1:1,000). Nuclei were stained blue with DAPI; FIH-1/HIF-1AN was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: FIH-1/HIF-1AN Antibody (23GB6385)[NBP3-51394] -
Flow cytometric analysis of FIH-1/HIF-1ANexpression in HeLa cells using FIH-1/HIF-1AN antibody (1:2,000). Green, isotype control; red, HIF1AN.
Western Blot: FIH-1/HIF-1AN Antibody (23GB6385)[NBP3-51394] -
Western blotting analysis using FIH-1/HIF-1AN antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for FIH-1/HIF-1AN Antibody (23GB6385)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FIH-1/HIF-1AN
Long Name
Factor Inhibiting HIF-1
Alternate Names
FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase
Gene Symbol
HIF1AN
Additional FIH-1/HIF-1AN Products
Product Documents for FIH-1/HIF-1AN Antibody (23GB6385)
Product Specific Notices for FIH-1/HIF-1AN Antibody (23GB6385)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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